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Ultrathin sections (80 nm thick) were cut with an ultramicrotome (Leica, EMUC6, Germany), and then stained with uranyl acetate and lead citrate and finally examined by a Tecnai G 2 Spirit TWIN transmission electron microscope (FEI, Hillsboro, OR, USA)

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After recording the movements of the mice for 5 min, the percentage time spent in the open arm and the number of entries into it were analyzed using the Noldus Ethovision ® XT (Noldus, Wageningen, The Netherlands)