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GSH triggers long-distance [Ca 2+ ] cyt transmission To further validate that GSH acts as a wound signal triggering long-distance plant defense signaling events, we applied GSH (with Glu and sorbitol as positive and negative controls, respectively) to leaf 1 of the GCaMP3 plants and visualized the systemic [Ca 2+ ] cyt in the whole seedlings (Fig

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The plate was finally scanned at 450 nm using a plate reader (Infinite 200 Pro MPlex, Tecan, Männedorf, Switzerland) after 50 μL of stop solution was added