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Adequate B12 levels are crucial for a healthy brain, supporting memory and cognitive function
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Serum biochemical analyses, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin (T-BIL), total proteins (TP), albumin, glucose, blood urea nitrogen (BUN), creatinine (CREA), uric acid (UA), TG, total cholesterol (T-CHO), high-density lipoproteins (HDL), low-density lipoproteins (LDL), calcium (Ca), magnesium (Mg), and phosphorus (P), were measured using Hitachi 7080 blood biochemistry analyzers (Hitachi Ltd., Taipei, Taiwan) and related reagents at the National Laboratory Animal Center [25]