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Despite the results from [53] being based on a limited number of patients, the acquired knowledge has encouraged further research aimed at testing the effects of creatine supplementation in several conditions including sarcopenia of ageing [54, 55], dystrophies [56, 57], mitochondrial myopathies [58, 59], COPD [60–62], and chronic heart failure (where phosphocreatine/ATP ratio is a stronger prognostic factor than the degree of impairment [63])
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Similarly, the levels of serum TNF-α, IL–6 and LPS, and fecal albumin levels were determined using the corresponding ELISA kit in accordance with the manufacturer’s protocols
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