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Researchers may examine: Binding affinity Stoichiometry Coordination geometry Copper exchange Competitive metal binding pH-dependent complex formation Stability of the peptide-metal complex Potential analytical methods include: UV-visible spectroscopy Mass spectrometry NMR spectroscopy Isothermal titration calorimetry Electron paramagnetic resonance ICP-MS or ICP-OES for elemental analysis These methods can help characterize how GHK-Cu differs from unbound GHK or free copper under defined experimental conditions